Increased frequency of ? cells with abnormal NKX6.1 expression in type 2 diabetes but not in subjects with higher risk for type 2 diabetes

Abstract Background NKX6.1 is a transcription factor for insulin, as well marker ? cell maturity. Abnormal expression in cells, such translocation from the nucleus to cytoplasm or lost expression, has been shown dedifferentiation. Methods We obtained pancreatic sections organ donors and immunofluorescence staining with insulin was performed characterize subjects without type 2 diabetes mellitus (T2DM). Results Our results showed that cells but no nucleic (NKX6.1 Nuc- Ins + ), cytoplasmic cyt ? ) were significantly increased T2DM positively correlated glycated hemoglobin (HbA1c), indicating elevated dedifferentiation inactivation T2DM. To investigate whether initiated higher risks T2DM, we next analyzed association between ?-cell level ND different ages, body mass index, HbA1c. The absolute number percentage of dedifferentiated did not change advanced aging, obesity, modest hyperglycemia, might mainly occur after diagnosed. Conclusion suggested changed diabetic subjects, evidenced by cells. This abnormality more frequently risk suggesting be secondary pathological changes